Contact : Nicolas ROCHEREAU Phone : +33 4 77 42 14 67
e-mail : rochereau.nicolas@gmail.com
Keywords : animal models, immunomonitoring, preclinical, clinical
Aims of “Immuno-monitoring of preclinical and clinical vaccine trial”:
Practical work will teach the students how to monitor humoral and cellular immune response in mice, how to measure immune diversity. Practical immunology includes multi-parameter flow cytometry analysis, elispot assay, elisa, route of administration in mice, mouse immunology.
1. Methods of immunisation in mice, uptake of organs and secretions
2. Multiparameter flow cytometry
3. In vivo imaging
4. Monitoring of B cell responses (elispot, antibody titers)
5. Monitoring of T cells (cytometry, polyfunctionality, elispot)
6. Transcriptomic, Proteomic and Glyconomics tools of the measure of vaccine efficiency
7. New methods as the measure of immune diversity
8. Management of samples for vaccine trial (AQ)
9. Choice of the good animal model and correlate of protection or efficiency
Speakers:
Teaching Staff Immunology from University of Jean Monnet Saint-Etienne directed by J. Lisfranc.
Conferences concerning news items will be organized. Speakers will be invited based on their excellence.
Practical training for preclinical immunomonitoring will be done with the CEA in Fontenay aux Roses (2 days) and University of Jean Monnet Saint-Etienne.
